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(A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and <t>anti-EXP1</t> antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.
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(A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and <t>anti-EXP1</t> antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.
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(A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and <t>anti-EXP1</t> antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.
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(A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and <t>anti-EXP1</t> antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.
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(A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and <t>anti-EXP1</t> antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.
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(A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and <t>anti-EXP1</t> antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.
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(A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and <t>anti-EXP1</t> antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.
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Image Search Results


(A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and anti-EXP1 antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.

Journal: bioRxiv

Article Title: A Plasmodium lipase is critical for the disruption of the parasitophorous vacuole membrane and egress of hepatic merozoites

doi: 10.1101/2025.06.02.657352

Figure Lengend Snippet: (A) Infected HepG2 cultures fixed at 65 hpi, were immunostained with the PVM markers anti-UIS4 and anti-EXP1 antibodies. Nuclei were stained with Hoechst. ( B and C) Intact and ruptured PVMs were quantified, and we detected a significantly greater number of EEFs with intact PVMs in UIS28 KO than in WT GFP immunostained with anti-UIS4 (****P<0.0001) and anti-EXP1 (**P=0.0068). The data were obtained from multiple coverslips and are presented as the mean ± SD. Statistical significance was determined by one-way ANOVA. (D) Infected cultures fixed at 65 hpi were immunostained with an anti-β-actin antibody. Nuclei were stained with Hoechst. Different types of actin localization patterns (condensed/dotted, absent, or associated with the plasma membrane) in infected cells were quantified. (E) Quantification of the actin localization pattern in infected cells. We detected a greater percentage of plasma membrane-associated actin in KO parasites than in WT parasites (*P=0.0162, Students t test). The other actin patterns were similar in both groups. A total of 72 (WT GFP) and 112 (UIS28 KO) EEFs were analyzed. Data were pooled from two independent experiments and are shown as the mean ± SD.

Article Snippet: KLH-conjugated peptides of EXP1 (KNKHGKTGSKNVIKKP-Cys) was synthesized by GL Biochem (Shanghai) Ltd.

Techniques: Infection, Staining, Clinical Proteomics, Membrane